Eglė Mikulionytė was born in 1965. After graduating from Juozas Naujalis Music School (currently Juozas Naujalis Music Gymnasium), the actress went to Syktyvkar, where she danced at Komi Republic State Opera and Ballet Theatre. After that she studied at the Lithuanian Academy of Music (currently the Lithuanian Academy of Music and Theatre) and since 1990 she has been working at the Lithuanian National Academic Drama Theatre (currently the Lithuanian National Drama Theatre). In 2008 the actress became the head of Jaja Theatre. Currently, Mikulionytė is an actress at the National Kaunas Drama Theatre.